Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)-19] (ab109735)


  • Product nameAnti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)-19]
    See all NAK/TBK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2867(2)-19] to NAK/TBK1 (phospho S172)
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NAK/TBK1 aa 150-250 (phospho S172). The exact sequence is proprietary.
    Database link: Q9UHD2

  • Positive control
    • HeLa, U937, HepG2, and 293T cell lysates, Human testis tissue, HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab109735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

For unpurified use at 1/1000 - 1/2000.

IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols

For unpurified use at 1/400.

Flow Cyt 1/90.

For unpurified use at 1/50.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionSerine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
  • Tissue specificityUbiquitous with higher expression in testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC antibody
    • FLJ11330 antibody
    • NAK antibody
    • NF kappa B activating kinase antibody
    • NF kB activating kinase antibody
    • NF-kappa-B-activating kinase antibody
    • Serine/threonine protein kinase TBK 1 antibody
    • Serine/threonine protein kinase TBK1 antibody
    • Serine/threonine-protein kinase TBK1 antibody
    • T2K antibody
    • TANK binding kinase 1 antibody
    • TANK-binding kinase 1 antibody
    • TBK 1 antibody
    • Tbk1 antibody
    • TBK1_HUMAN antibody
    see all

Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)-19] images

  • Predicted band size : 84 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: NAK/TBK1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109735 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab109735 was shown to recognize NAK/TBK1 when NAK/TBK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and NAK/TBK1 knockout samples were subjected to SDS-PAGE. ab109735 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)-19] (ab109735) at 1/4000 dilution + HeLa cell lysate at 10 µg

    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size : 84 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue using ab109735 at a dilution of 1/250.

  • ab109735 staining NAK/TBK1 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/800). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

  • Overlay histogram showing HeLa cells stained with ab109735 (red line) at 1/90 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

  • Overlay histogram showing HeLa cells stained with ab109735 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109735, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)-19] (ab109735) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size : 84 kDa

References for Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)-19] (ab109735)

This product has been referenced in:
  • Wei C  et al. Elevated expression of TANK-binding kinase 1 enhances tamoxifen resistance in breast cancer. Proc Natl Acad Sci U S A 111:E601-10 (2014). Read more (PubMed: 24449872) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (12% page)
Sample Human Cell lysate - whole cell (epithelioid pancreatic carcinoma - panc-1 atcc cel)
Specification epithelioid pancreatic carcinoma - panc-1 atcc cel
Treatment mock transfected - empty vector
Blocking step Milk as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Submitted Jan 17 2014