Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)

Overview

  • Product nameAnti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)]
    See all NAK/TBK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172)
  • SpecificityThis antibody only detects NAK/TBK1 phosphorylated at serine 172.
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human NAK/TBK1 .
    (Peptide available as ab206124)

  • Positive control
    • HeLa cell lysate, treated with Calyculin A.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab109272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).Can be blocked with NAK/TBK1 (phospho S172) peptide (ab206124).
IP 1/10 - 1/100.
  • Application notesIs unsuitable for ICC.
  • Target

    • FunctionSerine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
    • Tissue specificityUbiquitous with higher expression in testis.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC 2.7.11.1 antibody
      • FLJ11330 antibody
      • NAK antibody
      • NF kappa B activating kinase antibody
      • NF kB activating kinase antibody
      • NF-kappa-B-activating kinase antibody
      • Serine/threonine protein kinase TBK 1 antibody
      • Serine/threonine protein kinase TBK1 antibody
      • Serine/threonine-protein kinase TBK1 antibody
      • T2K antibody
      • TANK binding kinase 1 antibody
      • TANK-binding kinase 1 antibody
      • TBK 1 antibody
      • Tbk1 antibody
      • TBK1_HUMAN antibody
      see all

    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] images

    • All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution

      Lane 1 : Untreated NIH/3T3 (mouse embryo) whole cell lysate
      Lane 2 : NIH/3T3 (human cervix adenocarcinoma) treated with 10mM pervanadate for 30 minutes whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 84 kDa

      Blocking buffer and diluting buffer: 5% NFDM /TBST

    • All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma) treated with Calyculin A whole cell lysate
      Lane 2 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 84 kDa
      Additional bands at : 84 kDa. We are unsure as to the identity of these extra bands.

      Blocking buffer and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with Calyculin A

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa

    References for Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)

    ab109272 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (human colon lysates)
    Loading amount 50 µg
    Specification human colon lysates
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 19 2012

    Thank you for your recent telephone call. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    It has taken a little time to obtain more information regarding the WB testing protocol and I appreciate ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"