The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 20 µg/ml.
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 134 kDa.
FunctionAs a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions.
Sequence similaritiesBelongs to the NLRP family. Contains 13 LRR (leucine-rich) repeats. Contains 1 NACHT domain. Contains 1 pyrin domain.
Cellular localizationMitochondrion. Nucleus, nucleolus. Cytoplasm. In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages. From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions (By similarity).