Overview

  • Product name
  • Description
    Rabbit polyclonal to NALP6
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 167-197 of Human NALP6 (NP_612202.1).

  • Positive control
    • Human placenta tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 99 kDa.

Target

  • Function
    As the sensor component of the NLRP6 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP6, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. The precise NLRP6 activation stimulus has not been identified yet (By similarity) (PubMed:12387869). Essential for gut mucosal self-renewal and proliferation. Maintains intestinal homeostasis and a healthy intestinal microbiota. This function is, at least partially, mediated by IL18, and not IL1B, produced by nonhematopoietic cells. Influences intestinal barrier function and microbial homeostasis through the regulation of goblet cell mucus secretion. Acts by promoting autophagy in goblet cells, an essential step for mucus granule exocytosis. Its role in goblet cell physiology is inflammasome-dependent, but IL1B- and IL18-independent. During systemic bacterial infections, may negatively regulate inflammatory signaling and inhibit the influx of monocytes and neutrophils to the circulation and to the peritoneum. May promote peripheral nerve recovery following injury via an inflammasome-independent mechanism.
  • Tissue specificity
    Expressed in peripheral blood leukocytes, predominantly in granulocytes and, at lower levels, in CD4(+) and CD8(+) T-cells. Expressed in colonic myofibroblasts (at protein level) (PubMed:21593405).
  • Sequence similarities
    Belongs to the NLRP family.
    Contains 5 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
    Contains 1 pyrin domain.
  • Cellular localization
    Cytoplasm. Inflammasome. Cell membrane. Nucleus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Angiotensin II/vasopressin receptor antibody
    • AVR antibody
    • CLR11.4 antibody
    • NACHT, leucine rich repeat and PYD containing 6 antibody
    • NACHT, LRR and PYD containing protein 6 antibody
    • NACHT, LRR and PYD domains-containing protein 6 antibody
    • NAVR antibody
    • NAVR/AVR antibody
    • NLR family, pyrin domain containing 6 antibody
    • Nlrp6 antibody
    • NLRP6_HUMAN antibody
    • Nucleotide binding oligomerization domain, leucine rich repeat and pyrin domain containing 6 antibody
    • PAN3 antibody
    • PYPAF5 antibody
    • PYRIN containing APAF1 like protein 5 antibody
    • PYRIN-containing APAF1-like protein 5 antibody
    see all

Anti-NALP6 antibody images

  • Anti-NALP6 antibody (ab116007) at 1/100 dilution + Human placenta tissue lysate at 35 µg

    Predicted band size : 99 kDa

References for Anti-NALP6 antibody (ab116007)

ab116007 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
10 ug/ml azithromycin
Specification
THP-1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Apr 27 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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