Overview

  • Product nameAnti-Nanog antibody
    See all Nanog primary antibodies
  • Description
    Rabbit polyclonal to Nanog
  • SpecificityAt least two isoforms of Nanog are known to exist; ab106465 will detect both.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 19 amino acid synthetic peptide near the center of Human Nanog (EAW88651).

  • Positive control
    • Human spleen tissue lysate; Human spleen tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab106465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.

Target

  • FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similaritiesBelongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody images

  • Lane 1 : Anti-Nanog antibody (ab106465) at 1 µg/ml
    Lane 2 : Anti-Nanog antibody (ab106465) at 2 µg/ml

    Lane 1 : Human spleen tissue lysate
    Lane 2 : Human spleen tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 35 kDa
  • ab106465, at 5 µg/ml, staining Nanog in Human spleen tissue by Immunohistochemistry.
  • Immunofluorescence of NANOG in Human Spleen cells using ab106465 at 20 ug/ml.

References for Anti-Nanog antibody (ab106465)

This product has been referenced in:
  • Anchan RM  et al. Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells. PLoS One 9:e115106 (2014). ICC/IF ; Mouse . Read more (PubMed: 25517354) »

See 1 Publication for this product

Product Wall

I am sorry to hear that you have been experiencing problems with this antibody. The antibody was delivered on Friday and was received by you on Monday. Now not storing the products as recommended on the datasheet actually voids the Abpromise guarantee ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"