- Datasheet
- Specific References (5)
- Protocols
Overview
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Product nameAnti-Nanog antibody
See all Nanog primary antibodies -
DescriptionRabbit polyclonal to Nanog
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
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Species reactivityReacts with: Mouse
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Immunogen
A region between residue 250 and the C-terminus (residue 305) of Mouse Nanog using the numbering given in entry NP_079141.2
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Positive control
- Whole cell lysate from mouse embryonic stem cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab70482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 20331356 | |
| IHC-P | 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
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Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
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Sequence similaritiesBelongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 71950 Mouse
- SwissProt: Q80Z64 Mouse
- Unigene: 6047 Mouse
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Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Nanog antibody (ab70482)Image courtesy of an anonymous Abreview.ab70482 staining Nanog in murine embryonic stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.05% Triton X-100 and blocked using 10% serum for 2 hours at 20°C. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution. -
Western blot - Nanog antibody (ab70482)All lanes : Anti-Nanog antibody (ab70482) at 0.1 µg/ml
Lane 1 : Whole cell lysate
from mouse embryonic stem cells at 15 µg
Lane 2 : Whole cell lysate
from mouse embryonic stem cells at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 39 kDa (why is the actual band size different from the predicted?)
Exposure time: 12 seconds -
Immunoprecipitation - Nanog antibody (ab70482)Detection of Mouse Nanog by Western Blot of Immunoprecipitation with whole cell lysate (1mg) from mouse embryonic stem cells and ab 70482 at 2µg/mg lysate. Lane 1: ab70482, Lane 2: rabbit IgG control Immunoprecipitated Nanog was blotted using an anti-Nanog antibody at 0.1µg/ml. -
Western blot - Nanog antibody (ab70482)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Nanog antibody (ab70482) at 1/1000 dilution (for 16 hours at 4°C)
Lane 1 : Murine Embryonic Fibroblasts (MEFs)
Lane 2 : Murine Embryonic Stem (ES) Cells
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : An HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 40 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minute
Blocking Step: 3% BSA for 1 hour at 20°C -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab70482)This image is courtesy of an Abreview submitted by Carl Hobbsab70482 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with HIER buffer. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab70482 at 1/750 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Fritz AL et al. cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming. Mol Ther 23:952-963 (2015). Read more (PubMed: 25666918) »
- O'Reilly LP et al. An unexpected role for the clock protein timeless in developmental apoptosis. PLoS One 6:e17157 (2011). WB ; Mouse . Read more (PubMed: 21359199) »
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