Overview

  • Product nameAnti-Nanog antibody
    See all Nanog primary antibodies
  • Description
    Rabbit polyclonal to Nanog
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    A region between residue 250 and the C-terminus (residue 305) of Mouse Nanog using the numbering given in entry NP_079141.2

  • Positive control
    • Whole cell lysate from mouse embryonic stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20331356
IHC-P 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similaritiesBelongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody images

  • ab70482 staining Nanog in murine embryonic stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.05% Triton X-100 and blocked using 10% serum for 2 hours at 20°C. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.

    See Abreview

  • All lanes : Anti-Nanog antibody (ab70482) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from mouse embryonic stem cells at 15 µg
    Lane 2 : Whole cell lysate from mouse embryonic stem cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 seconds
  • Detection of Mouse Nanog by Western Blot of Immunoprecipitation with whole cell lysate (1mg) from mouse embryonic stem cells and ab 70482 at 2µg/mg lysate. Lane 1: ab70482, Lane 2: rabbit IgG control Immunoprecipitated Nanog was blotted using an anti-Nanog antibody at 0.1µg/ml.
  • All lanes : Anti-Nanog antibody (ab70482) at 1/1000 dilution (for 16 hours at 4°C)

    Lane 1 : Murine Embryonic Fibroblasts (MEFs)
    Lane 2 : Murine Embryonic Stem (ES) Cells

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 35 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab70482 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with HIER buffer. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab70482 at 1/750 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

References for Anti-Nanog antibody (ab70482)

This product has been referenced in:
  • O'Reilly LP  et al. An unexpected role for the clock protein timeless in developmental apoptosis. PLoS One 6:e17157 (2011). WB ; Mouse . Read more (PubMed: 21359199) »
  • Kinney SM  et al. Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes. J Biol Chem 286:24685-93 (2011). WB ; Mouse . Read more (PubMed: 21610077) »

See all 4 Publications for this product

Product Wall



The immunogen used to produce ab70482 is based on the mouse nanog sequence. The exact immunogen sequence is proprietary however.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse ES Cells)
Loading amount 50 µg
Specification Mouse ES Cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Utkarsh Kapoor

Verified customer

Submitted Dec 31 2012

Thank you for contacting us.

1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

or ab96879; http:...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Teratoma)
Specification Teratoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: HIER
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 12 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryonic)
Specification Embryonic
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X added to blocking solution
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Embryonic)
Loading amount 50 µg
Specification Embryonic
Gel Running Conditions Reduced Denaturing (15%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jul 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"