• Product nameAnti-Nanog antibody
    See all Nanog primary antibodies
  • Description
    Mouse monoclonal to Nanog
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus Monkey
  • Immunogen

    Recombinant fragment: TRTVFSSTQL CVLNDRFQRQ KYLSLQQMQE LSNILNLSYK QVKTWFQNQR MKSKRWQKNN WPKNSNGVTQ KASAPTYPSL YSSYHQGCLV NPTGNLPM, corresponding to amino acids 98-195 of Human Nanog (NP_079141) with a proprietary tag.

  • Positive control
    • HeLa nuclear extract.



Our Abpromise guarantee covers the use of ab89500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged Nanog is approximately 0.3ng/ml when used as a capture antibody.


  • FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similaritiesBelongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody images

References for Anti-Nanog antibody (ab89500)

This product has been referenced in:
  • Huang ZJ  et al. Reduced tumorigenicity and drug resistance through the downregulation of octamer-binding protein 4 and Nanog transcriptional factor expression in human breast stem cells. Mol Med Rep 11:1647-54 (2015). WB ; Human . Read more (PubMed: 25405855) »

See 1 Publication for this product

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