• Product name
    Anti-Nanog antibody (Biotin)
    See all Nanog primary antibodies
  • Description
    Rabbit polyclonal to Nanog (Biotin)
  • Conjugation
  • Specificity
    ab84231 allows the detection of at least 0.2 – 0.4 ng/well of recombinant human Nanog in ELISA assays and 1.5 - 3.0 ng/lane in Western blot, under either reducing or non-reducing conditions.
  • Tested applications
    Suitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human Nanog

  • Positive control
    • Recombinant human Nanog.



Our Abpromise guarantee covers the use of ab84231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
  • Application notes
    ELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well).
    sELISA: Use at a concentration of 0.25 - 1 µg/ml (100 µl/well).
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 35 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
    • Tissue specificity
      Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
    • Sequence similarities
      Belongs to the Nanog homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Developmental stage
      Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all

    References for Anti-Nanog antibody (Biotin) (ab84231)

    This product has been referenced in:
    • Chikkaveeraiah BV  et al. Ultrasensitive nanostructured immunosensor for stem and carcinoma cell pluripotency gatekeeper protein NANOG. Nanomedicine (Lond) 7:957-65 (2012). Read more (PubMed: 22394186) »

    See 1 Publication for this product

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