Anti-Nanog antibody [NNG-811] (ab62734)
Key features and details
- Mouse monoclonal [NNG-811] to Nanog
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Nanog antibody [NNG-811]
See all Nanog primary antibodies -
Description
Mouse monoclonal [NNG-811] to Nanog -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length human Nanog.
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Positive control
- Extracts of NT2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Purified Immunoglobulin -
Clonality
Monoclonal -
Clone number
NNG-811 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
Target
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Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
Images
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3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 µg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
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Lane 1 : Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/ml
Lane 2 : Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/ml
Lane 3 : Negative control – no primary antibody
All lanes : Cell extract of NT2 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Nanog was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell extract using ab62734.
Lane 1: 5 μg ab62734 IP in NT2/D1 whole cell extract.
Lane 2: 10 μg ab62734 IP in NT2/D1 whole cell extract.
Lane 3: Negative control without cell extract.
Lane 4: NT2/D1 whole cell extract (input).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab62734 has been referenced in 40 publications.
- Lawrence ML et al. Human iPSC-derived renal organoids engineered to report oxidative stress can predict drug-induced toxicity. iScience 25:103884 (2022). PubMed: 35243244
- Oldershaw RA et al. Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype. Biomedicines 10:N/A (2022). PubMed: 36551899
- Zeidler M et al. NOCICEPTRA: Gene and microRNA Signatures and Their Trajectories Characterizing Human iPSC-Derived Nociceptor Maturation. Adv Sci (Weinh) 8:e2102354 (2021). PubMed: 34486248
- Lv X et al. Curcumol inhibits malignant biological behaviors and TMZ-resistance in glioma cells by inhibiting long noncoding RNA FOXD2-As1-promoted EZH2 activation. Aging (Albany NY) 13:24101-24116 (2021). PubMed: 34739394
- Merkert S et al. Generation of an induced pluripotent stem cell line (MHHi018-A) from a patient with Cystic Fibrosis carrying p.Asn1303Lys (N1303K) mutation. Stem Cell Res 44:101744 (2020). PubMed: 32220772