Overview

  • Product nameAnti-Nanog antibody [PR-2E11]
    See all Nanog primary antibodies
  • Description
    Mouse monoclonal [PR-2E11] to Nanog
  • Tested applicationsSuitable for: Indirect ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human Nanog expressed in E. coli and refolded to soluble form.

  • Positive control
    • I-ELISA: immobilized Nanog immunogen WB: Nanog recombinant protein, HEK-293-Nanog transfectant cell lysate, NTERA-2 cell lysate, 2102 cell lysate
  • General notesThis product is for Research Use Only and not to be used for any commercial resale purpose, including, but not limited to, using the product as part of any developed assay technology such as ELISA, ELISPOT, or Multiplex assay that is sold to another end-user.

Properties

Applications

Our Abpromise guarantee covers the use of ab95334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1000.
WB 1/1000. Predicted molecular weight: 35 kDa.

Target

  • FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similaritiesBelongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Anti-Nanog antibody [PR-2E11] images

  • All lanes : Anti-Nanog antibody [PR-2E11] (ab95334) at 0.1 µg/ml

    Lane 1 : Nanog recombinant protein at 0.1 µg
    Lane 2 : Untransfected HEK-293 cell lysate at 20 µg
    Lane 3 : HEK-293-Nanog transfectant cell lysate at 20 µg
    Lane 4 : NTERA-2 cell lysate at 20 µg
    Lane 5 : 2102 cell lysate at 20 µg


    Predicted band size : 35 kDa
  • Indirect ELISA detection of immobilized Nanog immunogen with titrated amounts of ab95334. No binding observed to irrelevant (SOX2) protein.

References for Anti-Nanog antibody [PR-2E11] (ab95334)

ab95334 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"