Anti-NOS-1 antibody (ab106535)
Key features and details
- Rabbit polyclonal to NOS-1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-NOS-1 antibody -
Description
Rabbit polyclonal to NOS-1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Human NOS-1 (internal sequence).
Database link: Q8WY41 -
Positive control
- WB: Rat brain lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa.
|
Notes |
---|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 30 kDa. |
Target
-
Function
May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. -
Tissue specificity
Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness. -
Sequence similarities
Belongs to the nanos family.
Contains 1 nanos-type zinc finger. -
Developmental stage
Fetal ovary and fetal testis (at protein level). -
Domain
The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells. - Information by UniProt
-
Database links
-
Alternative names
- EC Rep1a antibody
- EC_Rep1a antibody
- NANO1_HUMAN antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab106535 has not yet been referenced specifically in any publications.