The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 2.5 µg/ml. (PMID 18223680)
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
Tissue specificityTestis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
Sequence similaritiesBelongs to the nanos family. Contains 1 nanos-type zinc finger.
Developmental stageFetal ovary and fetal testis (at protein level).
DomainThe Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein. The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
Cellular localizationCytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.