Overview

  • Product name
    Anti-Nanos Homologue 1 (NANOS1) antibody
    See all Nanos Homologue 1 (NANOS1) primary antibodies
  • Description
    Goat polyclonal to Nanos Homologue 1 (NANOS1)
  • Specificity
    This antibody is expected to recognise the longer isoform 1 only, not isoform 2.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    SARDGPPGKKLR

    , corresponding to amino acids 281-292 of Human Nanos Homologue 1 (NANOS1)

  • Positive control
    • Human testes lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab36163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
IHC-P
  • Application notes
    ELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 2.5 µg/ml. (PMID 18223680)
    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 30 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
    • Tissue specificity
      Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
    • Sequence similarities
      Belongs to the nanos family.
      Contains 1 nanos-type zinc finger.
    • Developmental stage
      Fetal ovary and fetal testis (at protein level).
    • Domain
      The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
      The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
    • Cellular localization
      Cytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • EC Rep1a antibody
      • EC_Rep1a antibody
      • NANO1_HUMAN antibody
      • NANOS 1 antibody
      • Nanos homolog 1 (Drosophila) antibody
      • Nanos homolog 1 antibody
      • NANOS1 antibody
      • Nos 1 antibody
      • NOS-1 antibody
      • Nos1 antibody
      • OTTHUMP00000046853 antibody
      • Rep 1a antibody
      • Rep1a antibody
      see all

    Images

    • Anti-Nanos Homologue 1 (NANOS1) antibody (ab36163) at 0.3 µg/ml (incubated for 1 hour) + Human Testis lysate in RIPA buffer. at 35 µg
      Developed using the ECL technique

      Predicted band size : 30 kDa
      Observed band size : 35 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Bonnomet A  et al. The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression. Oncogene 27:3692-9 (2008). IHC-P ; Human . Read more (PubMed: 18223680) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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