Anti-Nanos Homologue 1 (NANOS1) antibody (ab65203)

Overview

  • Product nameAnti-Nanos Homologue 1 (NANOS1) antibody
    See all Nanos Homologue 1 (NANOS1) primary antibodies
  • Description
    Rabbit polyclonal to Nanos Homologue 1 (NANOS1)
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide selected from the C-terminal region of human Nanos Homologue 1 (NANOS1)

  • Positive control
    • T47D cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab65203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 30 kDa).
ELISA 1/1000.

Target

  • FunctionMay act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.
  • Tissue specificityTestis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.
  • Sequence similaritiesBelongs to the nanos family.
    Contains 1 nanos-type zinc finger.
  • Developmental stageFetal ovary and fetal testis (at protein level).
  • DomainThe Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
    The N-terminal region and C-terminal zinc-finger RNA-binding domain are both necessary for interaction with SNAPIN.
  • Cellular localizationCytoplasm > perinuclear region. Cytoplasm. Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC Rep1a antibody
    • EC_Rep1a antibody
    • NANO1_HUMAN antibody
    • NANOS 1 antibody
    • Nanos homolog 1 (Drosophila) antibody
    • Nanos homolog 1 antibody
    • NANOS1 antibody
    • Nos 1 antibody
    • NOS-1 antibody
    • Nos1 antibody
    • OTTHUMP00000046853 antibody
    • Rep 1a antibody
    • Rep1a antibody
    see all

Anti-Nanos Homologue 1 (NANOS1) antibody images

  • Anti-Nanos Homologue 1 (NANOS1) antibody (ab65203) at 1/60 dilution + T47D cell line lysates at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab65203 at 1/50 dilution staining Nanos Homologue 1 in human breast carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

  • ab65203 at 1/50 dilution staining Nanos Homologue 1 in human Hela cells by Immunocytochemistry /Immunofluorescence. An Alexa-Fluor 546 conjugated donkey polyclonal to rabbit IgG was used as secondary.  

References for Anti-Nanos Homologue 1 (NANOS1) antibody (ab65203)

ab65203 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65203.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"