Overview

  • Product name
  • Description
    Rabbit polyclonal to Nanos3
  • Specificity
    ab70001 does not cross-react with either Nanos1 or Nanos2.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 16 amino acid peptide near the carboxy terminus of the human Nanos3 (GenBank accession no. P60323).

  • Positive control
    • Human brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).Can be blocked with Nanos3 peptide (ab176129).
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.
  • Tissue specificity
    Ovary, testis and brain (at protein level). In the ovaries, expressed during multiple stages of oogenesis, including primordial, primary, secondary and antral follicles with the highest expression in the oocytes. In the testis, expressed in germ cells, type A spermatogonia (SA), primary spermatocytes (S1), round spermatids (S3) and elongated spermatids.
  • Sequence similarities
    Belongs to the nanos family.
    Contains 1 nanos-type zinc finger.
  • Developmental stage
    Fetal ovary and fetal testis (at protein level).
  • Domain
    The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm > P-body. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with PUM2, EIF2S1 and TIAL1 in the stress granules. Co-localizes with DCP1A in the P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • NANO3_HUMAN antibody
    • Nanos homolog 3 antibody
    • NANOS3 antibody
    • NOS-3 antibody
    • Nos3 antibody
    see all

Images

  • Anti-Nanos3 antibody (ab70001) at 2 µg/ml + Human brain tissue lysate at 15 µg

    Predicted band size : 19 kDa
    Observed band size : 19 kDa
  • ab70001 at 2.5µg/ml staining Nanos3 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ICC/IF image of ab70001 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70001, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunofluorescence of Nanos3 in Human Brain cells using ab70001 at 20 ug/ml.

References

This product has been referenced in:
  • Liu C  et al. Activation of the germ-cell potential of human bone marrow-derived cells by a chemical carcinogen. Sci Rep 4:5564 (2014). ICC/IF ; Human . Read more (PubMed: 24998261) »
  • Wu X  et al. A NANOS3 mutation linked to protein degradation causes premature ovarian insufficiency. Cell Death Dis 4:e825 (2013). Read more (PubMed: 24091668) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, embryonic day 15.5)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, embryonic day 15.5
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Jul 05 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Testis)
Specification
Testis
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate + 0.05% Tween-20
Permeabilization
No
Blocking step
5% goat serum, 3% BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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