Overview

  • Product name
    Anti-NAP1 (C12orf30) antibody
    See all NAP1 (C12orf30) primary antibodies
  • Description
    Rabbit polyclonal to NAP1 (C12orf30)
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human NAP1 (C12orf30).

    (Peptide available as ab101294.)

  • Positive control
    • This antibody gave a positive signal in HeLa and HepG2 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 112 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp-Glu. May play a role in normal cell-cycle progression.
  • Sequence similarities
    Belongs to the MDM20/NAA25 family.
    Contains 4 TPR repeats.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • C12orf30 antibody
    • DKFZp667K2112 antibody
    • FLJ1308 antibody
    • MDM20 antibody
    • Mitochondrial distribution and morphology 20 antibody
    • Mitochondrial distribution and morphology protein 20 antibody
    • N(alpha)-acetyltransferase 25, NatB auxiliary subunit antibody
    • N-alpha-acetyltransferase 25, NatB auxiliary subunit antibody
    • N-terminal acetyltransferase B complex subunit MDM20 antibody
    • N-terminal acetyltransferase B complex subunit NAA25 antibody
    • Naa25 antibody
    • NAA25_HUMAN antibody
    • NAP1 antibody
    • NatB complex subunit MDM20 antibody
    • p120 antibody
    • TPR repeat-containing protein C12orf30 antibody
    see all

Images

  • All lanes : Anti-NAP1 (C12orf30) antibody (ab79490) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 112 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 46 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab79490 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79490, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 10µg/ml.

References

ab79490 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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