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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human NAP1L1.
(Peptide available as ab22418.)
Our Abpromise guarantee covers the use of ab33076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/250 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human NAP1L1 peptide (ab22418).|
|ICC/IF||Use a concentration of 1 µg/ml.|
For each lane, U2OS cells, either treated or not treated with ionizing radiation, were scraped from a 60mm dish and added to 75µl of 2X Laemmli buffer. 20µl of these samples were loaded into each lane. ab33076 recognizes a major band of approximately 52 kDa corresponding closely in size to NAP1L1.