Overview

  • Product name
    Anti-Nardilysin antibody
  • Description
    Rabbit polyclonal to Nardilysin
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 543-787 of Human Nardilysin.

  • Positive control
    • NT2D1 and Mouse kidney whole cell lysates; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 132 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    A member of the metalloproteinase family M16, nardilysin is most similar to insulin degrading enzyme, and the bacterial peptidase pitrilysin. It cleaves peptide substrates on the N terminus of arginine residues in dibasic pairs. Nardilysin was first described as a processing enzyme of somatostatin 1-28, which is cleaved at the Arg-Lys paired amino acid location. Nardilysin was named N arginine dibasic convertase (NRD convertase) to reflect this cleavage, although it was later shown that the activity was due to aminopeptidase B acting as a heterodimer with nardilysin. In addition to somatostatin 1-28, nardilysin cleaves dynorphin A and a-neoendorphin. Nardilysin was first detected in the brain and testis, and later in the heart, skeletal muscle, and in lesser amounts in most tissues and cell lines.
  • Database links
  • Alternative names
    • EC 3.4.24.61 antibody
    • hNRD1 antibody
    • hNRD2 antibody
    • N arginine dibasic convertase antibody
    • Nardilysin precursor antibody
    • NRD C antibody
    • NRD convertase antibody
    • NRD1 antibody
    see all

Images

  • Anti-Nardilysin antibody (ab154237) at 1/2000 dilution + NT2D1 whole cell lysate at 30 µg

    Predicted band size : 132 kDa
  • Anti-Nardilysin antibody (ab154237) at 1/1000 dilution + Mouse kidney whole cell lysate at 50 µg

    Predicted band size : 132 kDa
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling Nardilysin with ab154237 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

References

ab154237 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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