Overview

  • Product name
  • Description
    Rabbit polyclonal to NARF
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-239 of Human NARF (Uniprot: Q9UHQ1).

  • Positive control
    • A549, H1299, HCT116, MCF7 and Mouse muscle whole cell lysates; Mouse C2C12 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Relevance
    Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • DKFZp434G0420 antibody
    • FLJ10067 antibody
    • GC17P078009 antibody
    • IOP2 antibody
    • Iron only hydrogenase like protein 2 antibody
    • Nuclear prelamin A recognition factor antibody
    • Prenyl dependent prelamin A binding protein antibody
    see all

Images

  • All lanes : Anti-NARF antibody (ab153911) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HCT116 whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 51 kDa
  • Anti-NARF antibody (ab153911) at 1/1000 dilution + Mouse muscle whole cell lysate at 50 µg

    Predicted band size : 51 kDa
  • Immunohistochemical analysis of paraffin-embedded Mouse C2C12 xenograft tissue labeling NARF with ab153911 at 1/500 dilution.

References

ab153911 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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