Overview

  • Product name
  • Description
    Rabbit polyclonal to NARF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal amino acids 156-349 of Human NARF (Uniprot ID: Q9UHQ1).

  • Positive control
    • HeLa cells; A549 and MCF7 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab153936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • DKFZp434G0420 antibody
    • FLJ10067 antibody
    • GC17P078009 antibody
    • IOP2 antibody
    • Iron only hydrogenase like protein 2 antibody
    • Nuclear prelamin A recognition factor antibody
    • Prenyl dependent prelamin A binding protein antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells, labeling NARF with ab153936 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • All lanes : Anti-NARF antibody (ab153936) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : MCF7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    7.5% SDS PAGE

References

ab153936 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153936.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up