Overview

  • Product nameAnti-NARG1 antibody
    See all NARG1 primary antibodies
  • Description
    Rabbit polyclonal to NARG1
  • Tested applicationsSuitable for: ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (Human) from an internal sequence.

  • Positive control
    • Extracts from HUVEC cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionThe NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.
  • Tissue specificityExpressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.
  • Sequence similaritiesContains 8 TPR repeats.
  • Post-translational
    modifications
    Cleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment.
  • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5730450D16Rik antibody
    • 6330400I15 antibody
    • ASTBDN antibody
    • GA19 antibody
    • Gastric cancer antigen Ga19 antibody
    • MGC29428 antibody
    • mNAT1 antibody
    • N alpha acetyltransferase 15, NatA auxiliary subunit antibody
    • N terminal acetyltransferase antibody
    • N(alpha) acetyltransferase 15, NatA auxiliary subunit antibody
    • N-alpha-acetyltransferase 15 antibody
    • N-terminal acetyltransferase antibody
    • Naa15 antibody
    • NAA15_HUMAN antibody
    • NatA auxiliary subunit antibody
    • NATH antibody
    • NMDA receptor regulated 1 antibody
    • NMDA receptor regulated protein 1 antibody
    • NMDA receptor-regulated protein 1 antibody
    • Protein tubedown 1 antibody
    • Protein tubedown-1 antibody
    • Tbdn 1 antibody
    • Tbdn100 antibody
    • Transcriptional coactivator tubedown 100 antibody
    • tubedown-1 antibody
    see all

Anti-NARG1 antibody images

  • All lanes : Anti-NARG1 antibody (ab65107) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 101 kDa
    Observed band size : 101 kDa
  • ICC/IF image of ab65107 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65107, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NARG1 antibody (ab65107)

ab65107 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"