Overview

  • Product name
    Anti-NARS antibody [NARS2G5]
    See all NARS primary antibodies
  • Description
    Mouse monoclonal [NARS2G5] to NARS
  • Tested applications
    Suitable for: WB, IP, ICC, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment from Human NARS.

  • Positive control
    • HT-1080 cells and whole cell lysate, NIH 3T3 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab53473 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • Clonality
    Monoclonal
  • Clone number
    NARS2G5
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IP Use at 10 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.

Target

  • Sequence similarities
    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3010001M15Rik antibody
    • AA960128 antibody
    • AsnRS antibody
    • ASNS antibody
    • Asparagine tRNA ligase 1, cytoplasmic antibody
    • Asparagine tRNA ligase antibody
    • Asparagine--tRNA ligase antibody
    • Asparaginyl tRNA synthetase antibody
    • Asparaginyl-tRNA synthetase antibody
    • C78150 antibody
    • cytoplasmic antibody
    • EC 6.1.1.22 antibody
    • LRRGT00113 antibody
    • MGC116236 antibody
    • Nars antibody
    • NARS1 antibody
    • NRS antibody
    • SYNC_HUMAN antibody
    see all

Images

  • Anti-NARS antibody [NARS2G5] (ab53473) at 1/100 dilution + NIH 3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • ab53473 at 5ug used to immunoprecipitate RIPA lysate of HT-1080 cells (500µg). Western blot used ab53473 at 1/50 dilution with anti-mouse IgG at 1/2500 as secondary.
  • ab53473 at 1/10 dilution staining NARS in HT-1080 cells, by Immunocytochemistry. AlexaFluor®488 Goat Anti-mouse IgG at 1/200 used as secondary.
  • Anti-NARS antibody [NARS2G5] (ab53473) at 1/50 dilution + HT-1080 whole cell lysate at 25 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

References

ab53473 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab53473.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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