• Product name
  • Description
    Rabbit polyclonal to NASP
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 150 and 200 of human NASP (BAD96536.1)

  • Positive control
    • HeLa and 293T whole cell lysates.



Our Abpromise guarantee covers the use of ab72494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 86 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
    • Tissue specificity
      Isoform 1 is testis- and sperm-specific.
    • Sequence similarities
      Belongs to the NASP family.
      Contains 3 TPR repeats.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5033430J04Rik antibody
      • AI131596 antibody
      • AI317140 antibody
      • D4Ertd767e antibody
      • DKFZp547F162 antibody
      • Epcs32 antibody
      • FLB7527 antibody
      • FLJ31599 antibody
      • FLJ35510 antibody
      • MGC19722 antibody
      • MGC20372 antibody
      • MGC2297 antibody
      • MGC93869 antibody
      • NASP antibody
      • NASP_HUMAN antibody
      • nuclear autoantigenic sperm protein (histone binding) antibody
      • Nuclear autoantigenic sperm protein antibody
      • PRO1999 antibody
      • RP23 233B9.9 antibody
      see all

    Anti-NASP antibody images

    • All lanes : Anti-NASP antibody (ab72494) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size : 86 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)

      Exposure time : 10 seconds

    References for Anti-NASP antibody (ab72494)

    This product has been referenced in:
    • Li F  et al. Identification and analysis of signaling networks potentially involved in breast carcinoma metastasis to the brain. PLoS One 6:e21977 (2011). WB ; Human . Read more (PubMed: 21779361) »
    • Grad I  et al. The Molecular Chaperone Hsp90a Is Required for Meiotic Progression of Spermatocytes beyond Pachytene in the Mouse. PLoS One 5:e15770 (2010). WB ; Mouse . Read more (PubMed: 21209834) »

    See all 2 Publications for this product

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