Overview

  • Product nameAnti-NASP antibody
    See all NASP primary antibodies
  • Description
    Rabbit polyclonal to NASP
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide within Human NASP aa 626-675 (internal sequence). The exact sequence is proprietary.
    Sequence:

    KEAEGSSAEYKKEIEELKELLPEIREKIEDAKESQRSGNVAELALKATLV


    Database link: NP_689511

  • Positive control
    • HepG2 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRequired for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
  • Tissue specificityIsoform 1 is testis- and sperm-specific.
  • Sequence similaritiesBelongs to the NASP family.
    Contains 3 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5033430J04Rik antibody
    • AI131596 antibody
    • AI317140 antibody
    • D4Ertd767e antibody
    • DKFZp547F162 antibody
    • Epcs32 antibody
    • FLB7527 antibody
    • FLJ31599 antibody
    • FLJ35510 antibody
    • MGC19722 antibody
    • MGC20372 antibody
    • MGC2297 antibody
    • MGC93869 antibody
    • NASP antibody
    • NASP_HUMAN antibody
    • nuclear autoantigenic sperm protein (histone binding) antibody
    • Nuclear autoantigenic sperm protein antibody
    • PRO1999 antibody
    • RP23 233B9.9 antibody
    see all

Anti-NASP antibody images

  • Anti-NASP antibody (ab94779) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 85 kDa
    Gel concentration: 12%

    Note: the observed bank corresponds to isoform 2.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling NASP with ab94779 at 5µg/ml.

References for Anti-NASP antibody (ab94779)

ab94779 has not yet been referenced specifically in any publications.

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