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Rabbit monoclonal [EPR12827] to NASP
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NASP aa 50-150. The exact sequence is proprietary.
Database link: P49321
HeLa, Caco2, 293 or NTERA2/D1 lysate, 293 or HeLa cells.
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
1/100 - 1/250.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/40 - 1/60.
Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
Isoform 1 is testis- and sperm-specific.
Belongs to the NASP family.
Contains 3 TPR repeats.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Immunoprecipitation - Anti-NASP [EPR12827] antibody (ab181169)
Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
Flow Cytometry - Anti-NASP [EPR12827] antibody (ab181169)
Flow cytometry analysis of HeLa cells using ab181169 at a 1/40 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/40 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-NASP [EPR12827] antibody (ab181169)
Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
Western blot - Anti-NASP [EPR12827] antibody (ab181169)
All lanes :
Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilution
Lane 1 :
Lane 2 :
Lane 3 :
Lane 4 :
Lysates/proteins at 20 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"