Recombinant
RabMAb

Anti-NASP antibody [EPR12827] (ab181169)

Overview

  • Product name
    Anti-NASP antibody [EPR12827]
    See all NASP primary antibodies
  • Description
    Rabbit monoclonal [EPR12827] to NASP
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NASP aa 50-150. The exact sequence is proprietary.
    Database link: P49321

  • Positive control
    • HeLa, Caco2, 293 or NTERA2/D1 lysate, 293 or HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/40. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/40 - 1/60.

Target

  • Function
    Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
  • Tissue specificity
    Isoform 1 is testis- and sperm-specific.
  • Sequence similarities
    Belongs to the NASP family.
    Contains 3 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5033430J04Rik antibody
    • AI131596 antibody
    • AI317140 antibody
    • D4Ertd767e antibody
    • DKFZp547F162 antibody
    • Epcs32 antibody
    • FLB7527 antibody
    • FLJ31599 antibody
    • FLJ35510 antibody
    • MGC19722 antibody
    • MGC20372 antibody
    • MGC2297 antibody
    • MGC93869 antibody
    • NASP antibody
    • NASP_HUMAN antibody
    • nuclear autoantigenic sperm protein (histone binding) antibody
    • Nuclear autoantigenic sperm protein antibody
    • PRO1999 antibody
    • RP23 233B9.9 antibody
    see all

Images

  • Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.

  • Flow cytometry analysis of HeLa cells using ab181169 at a 1/40 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/40 dilution.

  • Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.

  • All lanes : Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : Caco2 lysate
    Lane 3 : 293 lysate
    Lane 4 : NTERA2/D1 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 85 kDa

    160KDa: tNASP
    62KDa: sNASP

References

ab181169 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181169.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up