Overview

  • Product nameAnti-NAT14 antibody
    See all NAT14 primary antibodies
  • Description
    Mouse monoclonal to NAT14
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment with a tag: MAPSHLSVRE MREDEKPLVL EMLKAGVKDT ENRVALHALT RPPALLLLAA ASSGLR, corresponding to amino acids 1-56 of human NAT14 (NP_065111). The tag sequence is not included here.

Properties

Applications

Our Abpromise guarantee covers the use of ab76846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBelongs to the camello family and contains 1 N-acetyltransferase domain. Expressed in K562 cells, HeLa cells and brain. Probable acetyltransferase that binds the 5'-GGACTACAG-3' sequence of coproporphyrinogen oxidase promoter. Able to activate transcription of a reporter construct in vitro.
    • Cellular localizationMembrane; Single pass membrane protein
    • Database links
    • Alternative names
      • K562 cell derived leucine zipper like protein 1 antibody
      • KLP1 antibody
      • N acetyltransferase 14 antibody
      • N acetyltransferase 14 (GCN5 related, putative) antibody

    Anti-NAT14 antibody images

    • Anti-NAT14 antibody (ab76846) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 22 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    References for Anti-NAT14 antibody (ab76846)

    ab76846 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Heart muscle)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Heart muscle
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative Paraformaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 14 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"