Anti-NAT8L antibody (ab76842)

Overview

  • Product name
    Anti-NAT8L antibody
  • Description
    Rabbit polyclonal to NAT8L
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide derived from the C terminal domain of mouse FLJ37478 protein

Properties

Applications

Our Abpromise guarantee covers the use of ab76842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150. (see Abreview)
IHC-Fr 1/50 - 1/200.
WB 1/100 - 1/1000. Predicted molecular weight: 33 kDa.

Target

  • Function
    Plays a role in the regulation of lipogenesis by producing N-acetylaspartate acid (NAA), a brain-specific metabolite. NAA occurs in high concentration in brain and its hydrolysis plays a significant part in the maintenance of intact white matter. Promotes dopamine uptake by regulating TNF-alpha expression. Attenuates methamphetamine-induced inhibition of dopamine uptake.
  • Tissue specificity
    Expressed in brain.
  • Sequence similarities
    Belongs to the camello family.
    Contains 1 N-acetyltransferase domain.
  • Cellular localization
    Cytoplasm. Membrane. Microsome membrane. Mitochondrion membrane. Rough endoplasmic reticulum membrane. Its enzymatic activity contribution is quantitatively larger in mitochondrial compartment than in extramitochondrial compartment.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110038O08Rik antibody
    • Camello-like protein 3 antibody
    • CML3 antibody
    • Hcml3 antibody
    • N acetyltransferase 8 like (GCN5 related, putative) antibody
    • N-acetylaspartate synthetase antibody
    • N-acetyltransferase 8-like protein antibody
    • NAA synthetase antibody
    • NAT8 like antibody
    • Nat8l antibody
    • NAT8L_HUMAN antibody
    • protein Shati antibody
    • Shati antibody
    see all

Images

  • ab76842 staining FLJ37478 in CHO cells transfected with mouse Nat8l cDNA by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/150 in 1% BSA in PBS and incubated with the sample for 1 hour at 25°C. The secondary antibody was Cy2® conjugated goat anti-rabbit polyclonal, diluted 1/200.

    See Abreview

  • All lanes : Anti-NAT8L antibody (ab76842) at 1/1000 dilution (in TBS + 0.05% Tween 20 for 1 hour at 22°C)

    Lane 1 : CHO-K1 cells expressing mouse Nat8L cDNA
    Lane 2 : CHO-K1 cells transfected with empty vector

    Lysates/proteins at 70 µg per lane.

    Secondary
    An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 33 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreveiw

    Blocking STep: 3% Milk for 30 minutes at 22 °C

    See Abreview

References

ab76842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CHO-K1 cells transfected with Nat8L cDNA)
Loading amount
70 µg
Specification
CHO-K1 cells transfected with Nat8L cDNA
Gel Running Conditions
Reduced Denaturing (10%, polyacrylamide)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (CHO cells transfected with mouse Nat8l cDNA)
Specification
CHO cells transfected with mouse Nat8l cDNA
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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