Native Clostridium difficile Toxin B protein (ab124001)


  • Product name
    Native Clostridium difficile Toxin B protein
  • Protein length
    Full length protein


  • Nature
  • Source
  • Amino Acid Sequence
    • Accession
    • Molecular weight
      270 kDa
    • Amino acids
      2 to 2366

Associated products


Our Abpromise guarantee covers the use of ab124001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications


    Functional Studies

  • Purity
    > 95 % SDS-PAGE.
    Clostridium difficile Toxin B has been highly purified.
  • Form
  • Additional notes

    This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.

    Reconstituted liquid can be stored at -80°C or -20°C to extend stability.

    Functional Studies Protocol

    1.1 Vero cells are plated out the night before on a 96-well plate at a concentration of 20K cells per well (in 100ml), then incubated at 37°C, 5% CO2. The following day they should be lightly confluent.
    1.2 Lyophilised toxin/toxoid is resuspended as per the instructions in the certificate of analysis, and diluted in duplicate to 0.1mg/ml in DMEM (200ml total) without FBS in the first well of a non-TC-treated 96-well plate. A serial 5-fold dilution is performed (40ul in 160ul) in the same media using a multichannel for 11 dilutions in total, leaving the 12th dilution as a media control.
    1.3 20ul of each dilution is then pipetted onto each well of the pre-prepared Vero cells and then incubated at 37°C, 5% CO2 for 24 hours.
    1.4 The following day, the cells are examined using an inverted phase contrast tissue culture microscope. Cells with active toxin on should be rounded, cells without significant toxicity should look like the control cells.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Please see notes section.

    Constituents: 1.19% HEPES, 5% Sucrose, 0.88% Sodium chloride

  • Reconstitution
    Reconstitute in 250µl sterile distilled water. Reconstituted liquid can be stored at -80°C or -20°C to extend stability.

General Info

  • Alternative names
    • C. difficile Toxin B
    • Clostridium difficile Toxin B
    • Cytotoxin B
    • tcdB
    • toxB
    • Toxin B
    see all
  • Relevance
    Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli.
  • Cellular localization


  • SDS-PAGE showing ab124001 at 270 kDa.


This product has been referenced in:
  • Hutton ML  et al. Bovine antibodies targeting primary and recurrent Clostridium difficile disease are a potent antibiotic alternative. Sci Rep 7:3665 (2017). Neutralising ; Clostridium difficile . Read more (PubMed: 28623367) »

See 1 Publication for this product

Customer reviews and Q&As

These products were purified from Clostridium difficile strain VPI10463 (which produces toxinotype 0 toxins). The toxins are active, each batch is tested by verocytotoxicity assay and demonstrated to be toxic to cells down to at least 5 ng/ml...

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Thank you for contacting us.

In response to your questions:

1)The toxin is guaranteed to be stable for at least 1 month after reconstitution, (we have data that shows 6 weeks is still fine).

2)The toxin can be frozen for l...

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Thank you for contacting us again.

I am delighted to send you the protocol of the cell toxicity assay with Vero cells

1 Verocycotoxicity Assay
1.1 Vero cells are plated out the night before on a 96-well plate a...

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I have contacted the lab regarding your question and here is their answer:

Yes, it is fully cytotoxic and has been quality controlled in a vero cell cytotoxicity assay.

Vielen Dank für Ihren Anruf.

Zu den Toxinen kann ich Ihnen folgende Informationen aus dem Labor weiterleiten:

Clostridium difficile Toxin A Protein ab123999und Clostridium difficile Toxin B Protein ab124001werden aus demClostri...

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