Native E. coli beta Galactosidase protein (ab79449)

Overview

Description

  • Nature
    Native
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Escherichia coli

Specifications

Our Abpromise guarantee covers the use of ab79449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% (w/v) Sucrose, 0.05M Potassium phosphate, pH 7.8

General Info

  • Alternative names
    • Beta D galactosidase
    • Beta gal
    • Beta galactosidase
    • ECK0341
    • JW0335
    • Lactase
    • lacZ
    see all
  • Relevance
    Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • Cellular localization
    Cytoplasmic

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us on Friday of last week. I am sorry for the delay in getting back to you.

As discussed over the phone I have looked into the activity of the beta Galactosidase protein (ab79449). We do not have precise activity data ...

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ab7981:

It is an active protein, the assay we use to determine activity is Beta galactosidase assay. We have not sequenced this product but it is sourced from E coli and should be similar to P00722 (http://www.uniprot.org/uniprot/P0...

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Thank you for your enquiry and interest in our products.

I can confirm that these two products (ab7981 and ab79449) are not His-tagged. I have checked our catalogue and currently we only sell non-tagged beta Galactosidase protein.

I...

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Thank you for your inquiry. I am afraid that these products, ab7981 and ab79449, does not contain a His tag. If there is anything else that I can help you with, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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