Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Additional sequence information
      Prepared from plasma

Specifications

Our Abpromise guarantee covers the use of ab90285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.
  • Applications

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm =1.46.

     

    The IgG fc fragment does not bind antigen; however, it does contain the classic antigenic determinants and biological activity with respect to cytotrophic reactions, binding of complement and catabolic rate control.

     

    Shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 and 2 by FDA approved tests.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.05% Sodium Azide
    Constituents: 50mM Tris HCl, 200mM Sodium chloride, pH 8.0

General Info

  • Relevance
    The Fc region is the constant region on an immunoglobulin molecule, the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens.

Images

  • 4-12% Bis-Tris NuPAGE Gel analysis of ab90285.

    Lanes 1-3 show 5, 10 and 15 μg, respectively; run under heated/reducing conditions.

    Lanes 4-6 show 5, 10 and 15 μg, respectively; not heated/non-reducing conditions.

References

This product has been referenced in:
  • Kisiswa L  et al. T-type Ca2+ channels are required for enhanced sympathetic axon growth by TNFa reverse signalling. Open Biol 7:N/A (2017). Read more (PubMed: 28100666) »
  • Cohen DJ  et al. Epithelial self-healing is recapitulated by a 3D biomimetic E-cadherin junction. Proc Natl Acad Sci U S A 113:14698-14703 (2016). Read more (PubMed: 27930308) »

See all 5 Publications for this product

Customer reviews and Q&As


We prepare IgG, FC Fragment from polyclonal IgG.

We do not know the glycosylation status of the IgG FC fragment as we do not test for this as part of our final QC analysis. We do not actively attempt to deglycosylate the FC as part of the purification procedure. I am including a general description o...

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Unfortunately, we do not have information on the structure of the protein.

For the recent batch we sell, ab90285 lot# GR90653 is 2.1 mg/ml.

Thank you for your reply.

I am confident that the reference ab90285 is a suitable negative control for ab108557.
Furthermore, I have just been informed about the price of the new Fc fragments which is $424.00 for 100µg, therefore I w...

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Thank you for contacting us.

The lab let me know that they have not measured the endotoxin levels for this product.
Also, they do not know whether or not it could be used a s a control in cell culture.

However, if you would li...

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Thank you for your reply.

While we don't have an exact figure for how sensitive the immunoelectrophoresis is, we do not believe the protein will contain 1% contamination with F(ab). I hope this helps, please let me know if you need any other ...

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Thank you for contactingus.

This Fc fragment protein shows no reaction to F(ab) on immunoelectrophoresis. We will guarantee this product to be free from F(ab) fragment contamination. Unfortunately, we do not currently have any other purified ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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