Overview

  • Product name
    Native KLH protein
  • Protein length
    Full length protein

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Accession
    • Species
      Megathura crenulata
    • Sequence
      DFGHSKKIRK NVHSLTAEEQ NSLRRAMDDL QDDKTRGGFQ QIAAFHGEPK WCPRPEAEKK FACCVHGMAV FPHWHRLLTV QGENALRKHG FTGGLPYWDW TRPMSALPHF VADPTYDDSV SSLEEDNPYS HGHIDSVGHD TTRAVRDDLY QSPGFGHYTD IAKQVLLALE QDDFCDFEVQ FEIAHNSIHA LVGGNEPYGM STLEYFLYDP IFFLHHSNTD RLWAIWQALQ KYRGKPYNTA NCAIVRHDTY RKPLQPFGLD SVINPDDETR EHSVPRDVFN YKDDFNYEYE SLNFNGLSIA QLDRELQRIK SHDRVFAGFL LHEIGQSALV KFYVCKHHVS DCDHYAGEFY ILGDEAEMPF AYDRVYKYEI SQALHDLDLH VGDNFHLKYE AFNLNGGSLG GVDLSQPSVI FEPAAGSHTA
    • Molecular weight
      48 kDa

Specifications

Our Abpromise guarantee covers the use of ab157012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Conjugation

  • Endotoxin level
    < 50.000 Eu/mg
  • Purity
    > 90 % Size exclusion.
    ab157012 was determined to be >90% pure as a mixture of high and low molecular weight forms and >50% pure as high molecular weight forms by size exclusion chromatography.
  • Form
    Lyophilised
  • Additional notes
    This preparation of chromatographically purified hemocyanin has been treated to reduce endotoxins for immunization protocols. Upon reconstitution with PBS a highly soluble solution is obtained for efficient and direct use for peptide coupling.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    Lyophilized from BES buffer containing MgSO4.

  • Reconstitution
    Soluble in PBS or other physiological buffer at 5-10 mg/ml. Note that material is supplied as gross weight. Protein content is =60%

General Info

  • Alternative names
    • Hemocyanin 2 c chain
    • Hemocyanin 2c chain
    • Hemocyanin A chain
    • Hemocyanin B chain
    • Keyhole limpet hemocyanin
    • KLH A
    • KLH B
    • KLH2 c
    • KLH2c
    • KLHA
    • KLHB
    see all
  • Relevance
    KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
  • Cellular localization
    Secreted protein; extracellular space

References

ab157012 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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