The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
< 50.000 Eu/mg
% Size exclusion. ab157012 was determined to be >90% pure as a mixture of high and low molecular weight forms and >50% pure as high molecular weight forms by size exclusion chromatography.
This preparation of chromatographically purified hemocyanin has been treated to reduce endotoxins for immunization protocols. Upon reconstitution with PBS a highly soluble solution is obtained for efficient and direct use for peptide coupling.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Lyophilized from BES buffer containing MgSO4.
Soluble in PBS or other physiological buffer at 5-10 mg/ml. Note that material is supplied as gross weight. Protein content is =60%
Hemocyanin 2 c chain
Hemocyanin 2c chain
Hemocyanin A chain
Hemocyanin B chain
Keyhole limpet hemocyanin
KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.
Secreted protein; extracellular space
has not yet been referenced specifically in any publications.
Publishing research using ab157012? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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