Anti-Natriuretic Peptide Receptor A antibody (ab14356)

Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab14356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
ICC/IF
Flow Cyt
IHC-Fr
  • Application notesFlow Cyt: 1/200 (PMID 18192083).
    ICC/IF: 1/300 (PMID 18192083).
    ICC: 1/200.
    IHC-P: 1/4000.
    IHC-Fr: Suggested working dilution of 1/300 (see PMID: 17043672).
    WB: 1/5000. Predicted molecular weight: 118 kDa. Can be blocked with Natriuretic Peptide Receptor A peptide (294-308) (ab28437). In the Tian et al. paper, the membrane was blocked in 20% non-fat milk and the primary and secondary antibody was also diluted in 10% non-fat milk to reduce the background.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionReceptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
    • Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
      Contains 1 guanylate cyclase domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylation of the protein kinase-like domain is required for full activation by ANP.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANP-A antibody
      • ANPa antibody
      • ANPR-A antibody
      • ANPRA antibody
      • ANPRA_HUMAN antibody
      • Atrial natriuretic peptide A type receptor antibody
      • Atrial natriuretic peptide receptor 1 antibody
      • Atrial natriuretic peptide receptor A antibody
      • Atrial natriuretic peptide receptor type A antibody
      • Atrionatriuretic peptide receptor A antibody
      • GC A antibody
      • GC-A antibody
      • Guanylate cyclase A antibody
      • Guanylate cyclase antibody
      • GUC2A antibody
      • GUCY2A antibody
      • Natriuretic peptide A type receptor antibody
      • Natriuretic peptide receptor A/guanylate cyclase A antibody
      • NPR-A antibody
      • NPR1 antibody
      • NPRA antibody
      see all

    Anti-Natriuretic Peptide Receptor A antibody images

    • ICC/IF image of ab14356 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14356, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Natriuretic Peptide Receptor A antibody (ab14356)

    This product has been referenced in:
    • You H & Laychock SG Long-term treatment with atrial natriuretic peptide inhibits ATP production and insulin secretion in rat pancreatic islets. Am J Physiol Endocrinol Metab 300:E435-44 (2011). WB ; Rat . Read more (PubMed: 20959527) »
    • Liu C  et al. Inhibition of dehydration-induced water intake by glucocorticoids is associated with activation of hypothalamic natriuretic peptide receptor-A in rat. PLoS One 5:e15607 (2010). IHC-FoFr ; Rat . Read more (PubMed: 21187974) »

    See all 7 Publications for this product

    Product Wall

    Application Flow Cytometry
    Sample Human Cell (HEK293)
    Permeabilization No
    Gating Strategy viability and PE
    Specification HEK293
    Preparation Cell harvesting/tissue preparation method: FACS buffer
    Sample buffer: PBS with 1% FBS
    Fixation None
    Username

    Abcam user community

    Verified customer

    Submitted Nov 23 2015

    Thank you for your enquiry which we have received through Labome.

    For more information regarding ab14356 Natriuretic Peptide Receptor A antibody, I can recommend to review the online datasheet. This will contain all the information we have r...

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    I received your voicemail and will give you a call. The authors of the recent Neuroscience paper have not replied to me. I examined the original reference: Hirsch JR et al. Cellular localization, membrane distribution, and possible function of guany...

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    Thank you for getting back to me. I am pleased that you have published your data. Thank you very much for getting back to me. I have added this publication to the references specific for this antibody.

    I'm sorry to hear you are having a problem with ab14356. Typically, an entirely black blot would mean that there is not enough blocking or far too much protein or antibody. A recent reviewer suggested that the antibody worked well although they did...

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    Thank you for your enquiry. I have found two papers that use membrane loading controls: 1. BiP, found on ER: www.jbc.org/cgi/content/full/278/36/34725 We have several antiBiP antibodies; I suggest ab2902 http://www.abcam.com/index.html?d...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human aortic endothelial cells)
    Loading amount 20 µg
    Specification human aortic endothelial cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Brian Van Ness

    Verified customer

    Submitted Jul 17 2006

    Thank you for your enquiry. I am pleased to report that I have clarified this issue. The immunogen that was used to raise Rabbit polyclonal to Natriuretic Peptide Receptor A (ab14356) was Natriuretic Peptide Receptor A peptide (294-308) (ab28437)...

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    Thank you for your patience. The originator of ab14356 has told me that the antibody was tested with the corresponding antigen for which it was made for. I'm afraid that I'm unable to obtain any more information at this time. Rat kidney membrane sample...

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    Thank you very much for your patience and for the details that you have provided. I'm currently working with the originator of this antibody and am waiting for some more information from them - in particular, what sort of lysate was used to charact...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"