Anti-Natriuretic Peptide Receptor A antibody (ab40817)

Overview

  • Product nameAnti-Natriuretic Peptide Receptor A antibody
    See all Natriuretic Peptide Receptor A primary antibodies
  • Description
    Rabbit polyclonal to Natriuretic Peptide Receptor A
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human Natriuretic Peptide Receptor A.

    (Peptide available as ab41439.)

  • Positive control
    • This antibody gave a positive signal in Kidney (Human) Tissue Lysate - adult normal tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab40817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 119 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionReceptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
  • Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylation of the protein kinase-like domain is required for full activation by ANP.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANP-A antibody
    • ANPa antibody
    • ANPR-A antibody
    • ANPRA antibody
    • ANPRA_HUMAN antibody
    • Atrial natriuretic peptide A type receptor antibody
    • Atrial natriuretic peptide receptor 1 antibody
    • Atrial natriuretic peptide receptor A antibody
    • Atrial natriuretic peptide receptor type A antibody
    • Atrionatriuretic peptide receptor A antibody
    • GC A antibody
    • GC-A antibody
    • Guanylate cyclase A antibody
    • Guanylate cyclase antibody
    • GUC2A antibody
    • GUCY2A antibody
    • Natriuretic peptide A type receptor antibody
    • Natriuretic peptide receptor A/guanylate cyclase A antibody
    • NPR-A antibody
    • NPR1 antibody
    • NPRA antibody
    see all

Anti-Natriuretic Peptide Receptor A antibody images

  • Anti-Natriuretic Peptide Receptor A antibody (ab40817) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 119 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab40817 stained human HEK 293 cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40817, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-Natriuretic Peptide Receptor A antibody (ab40817)

ab40817 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Jun 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"