The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml.
Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionReceptor for the C-type natriuretic peptide NPPC/CNP hormone. Has guanylate cyclase activity upon binding of its ligand. May play a role in the regulation of skeletal growth.
Involvement in diseaseDefects in NPR2 are the cause of acromesomelic dysplasia Maroteaux type (AMDM) [MIM:602875]. Acromesomelic chondrodysplasias are rare hereditary skeletal disorders characterized by short stature, very short limbs, and hand/foot malformations. The severity of limb abnormalities increases from proximal to distal with profoundly affected hands and feet showing brachydactyly and/or rudimentary fingers (knob-like fingers). AMDM is an autosomal recessive form characterized by axial skeletal involvement with wedging of vertebral bodies. In AMDM all skeletal elements are present but show abnormal rates of linear growth.
Sequence similaritiesBelongs to the adenylyl cyclase class-4/guanylyl cyclase family. Contains 1 guanylate cyclase domain. Contains 1 protein kinase domain.
Post-translational modificationsPhosphorylation of the protein kinase-like domain is required for full activation by CNP.
Atrial natriuretic peptide B type receptor antibody
Atrial natriuretic peptide receptor 2 antibody
Atrial natriuretic peptide receptor type B antibody
Atrionatriuretic peptide receptor B antibody
Guanylate cyclase B antibody
GUC 2B antibody
Natriuretic peptide receptor B antibody
Natriuretic peptide receptor B/guanylate cyclase B antibody
Anti-Natriuretic Peptide Receptor B antibody images
Immunocytochemistry/ Immunofluorescence - Anti-Natriuretic Peptide Receptor B antibody (ab55724)
ICC/IF image of ab55724 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55724, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43um..
IHC-P - Natriuretic Peptide Receptor B antibody (ab55724)
Natriuretic Peptide Receptor B antibody (ab55724) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human small Intestine.
Western blot - Natriuretic Peptide Receptor B antibody (ab55724)
Western blot against tagged recombinant protein immunogen using ab55724 Natriuretic Peptide Receptor B antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
Flow Cytometry-Anti-Natriuretic Peptide Receptor B antibody(ab55724)
Overlay histogram showing SH-SY5Y cells stained with ab55724 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55724, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
References for Anti-Natriuretic Peptide Receptor B antibody (ab55724)
has not yet been referenced specifically in any publications.
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