Anti-Natriuretic Peptide Receptor C antibody (ab14355)

Overview

  • Product name
    Anti-Natriuretic Peptide Receptor C antibody
    See all Natriuretic Peptide Receptor C primary antibodies
  • Description
    Rabbit polyclonal to Natriuretic Peptide Receptor C
  • Tested applications
    Suitable for: IHC-FoFr, ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide:

    QEEGLHSIYSFDET

    conjugated to poly Lysin, corresponding to amino acids 199-212 of Human Natriuretic Peptide Receptor C.

Properties

Applications

Our Abpromise guarantee covers the use of ab14355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/800. PubMed: 19878700
ICC 1/800.
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 64 kDa.

Target

  • Function
    Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
  • Sequence similarities
    Belongs to the ANF receptor family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANP C antibody
    • ANP-C antibody
    • ANPC antibody
    • ANPR-C antibody
    • ANPRC antibody
    • ANPRC_HUMAN antibody
    • Atrial natriuretic peptide C type receptor antibody
    • Atrial natriuretic peptide clearance receptor antibody
    • Atrial natriuretic peptide receptor 3 antibody
    • Atrial natriuretic peptide receptor type C antibody
    • Atrionatriuretic peptide receptor C antibody
    • C5orf23 antibody
    • FLJ14054 antibody
    • GUCY2B antibody
    • MGC22189 antibody
    • Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) antibody
    • Natriuretic peptide receptor C/guanylate cyclase C antibody
    • NPR 3 antibody
    • NPR C antibody
    • NPR-C antibody
    • Npr3 antibody
    • NPRC antibody
    see all

Images

  • ICC/IF image of ab14355 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14355, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

See all 5 Publications for this product

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