Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • Additional sequence informationpurified from natural source, either algae or cyanobacteria

Specifications

Our Abpromise guarantee covers the use of ab123473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    FRET

    Flow Cytometry

    Immunocytochemistry/ Immunofluorescence

  • FormLiquid
  • Additional notes

    Before use, centrifuge ab123472 suspension at 10 000g for 10 min at 4°C. Discard the supernatant and resuspend the pellet into the desired buffer. Desalt the sample using either Sephadex G-25 or dialysis. Abs/Em = 651/662 nm.

    The extinction coefficient of CL-APC at 650nm is 70,000 cm-1M-1.

     

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.

    pH: 7.00
    Note: Ammonium sulfate is around 40% for protein precipitation

General Info

  • Alternative names
    • apcB
    • Allophycocyanin alpha chain
    • Allophycocyanin beta chain
    • apcA
  • RelevanceAllophycocyanin is a protein from the light-harvesting phycobiliprotein family, along with phycocyanin, phycoerythrin and phycoerythrocyanin. It is an accessory pigment to chlorophyll. All phycobiliproteins are water soluble and therefore cannot exist within the membrane like carotenoids, but aggregate to form clusters that adhere to the membrane called phycobilisomes. Allophycocyanin absorbs and emits red light (650 & 660 nm max, respectively), is readily found in Cyanobacteria and red algae, and is a useful immunoassay tool. APC is made up of alpha and beta subunits and is present as a trimer (alpha beta)3. The trimer is unstable and susceptible to dissociation at low concentrations. The monomer, alpha beta, has a lower fluorescence quantum yield compared to the trimer and the maximal absorption is shifted to 620 nm. The chemically crosslinked APC (CL-APC) trimer is much more stable than the native protein, but still retains comparable spectroscopic properties. The extinction coefficient of CL-APC at 650 nm is 700,000 cm-1M-1.

References for Natural Allophycocyanin protein (ab123473)

ab123473 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab123473.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"