The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Before use, centrifuge ab123472 suspension at 10 000g for 10 min at 4°C. Discard the supernatant and resuspend the pellet into the desired buffer. Desalt the sample using either Sephadex G-25 or dialysis. Abs/Em = 651/662 nm.
The extinction coefficient of CL-APC at 650nm is 70,000 cm-1M-1.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
pH: 7.00 Note: Ammonium sulfate is around 40% for protein precipitation
Allophycocyanin alpha chain
Allophycocyanin beta chain
RelevanceAllophycocyanin is a protein from the light-harvesting phycobiliprotein family, along with phycocyanin, phycoerythrin and phycoerythrocyanin. It is an accessory pigment to chlorophyll. All phycobiliproteins are water soluble and therefore cannot exist within the membrane like carotenoids, but aggregate to form clusters that adhere to the membrane called phycobilisomes. Allophycocyanin absorbs and emits red light (650 & 660 nm max, respectively), is readily found in Cyanobacteria and red algae, and is a useful immunoassay tool.
APC is made up of alpha and beta subunits and is present as a trimer (alpha beta)3. The trimer is unstable and susceptible to dissociation at low concentrations. The monomer, alpha beta, has a lower fluorescence quantum yield compared to the trimer and the maximal absorption is shifted to 620 nm. The chemically crosslinked APC (CL-APC) trimer is much more stable than the native protein, but still retains comparable spectroscopic properties. The extinction coefficient of CL-APC at 650 nm is 700,000
References for Natural Allophycocyanin protein (ab123473)
has not yet been referenced specifically in any publications.
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