The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
One hemolytic unit will cause 50% lysis of a 1% suspension of rabbit red blood cells in phosphate buffered saline, pH 7.0, containing 1% bovine serum albumin after 30 min at 37 °C followed by refrigeration for 30 min at 4 °C.
Extinction Coefficient: E= 11 (280 nm)
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects. Derived from Staphylococcus aureus.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituent: 0.03% Sodium citrate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Soluble in deionized water (0.5 mg/ml), yielding a
colorless, faint hazy solution
Alpha-toxin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. Heptamer oligomerization and pore formation is required for lytic activity.
Secreted. Note: Secreted as a monomer. After oligomerization and pore formation, the complex is translocated across the bilayer, probably via the Gly-rich domain of each strand.
has not yet been referenced specifically in any publications.
Publishing research using ab192009? Please let us know so that we can cite the reference in this datasheet.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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