Natural Carcino Embryonic Antigen CEA protein (ab742)

Overview

Description

  • Nature
    Native
  • Source
    Native

Specifications

Our Abpromise guarantee covers the use of ab742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    SDS-PAGE denaturating : multiple protein bands 150 – 210 kDa
  • Applications

    ELISA

  • Form
    Liquid
  • Additional notes
    Blood samples from tissue donors were tested and found negative for HBsAg, HIV-1 and HIV-2 antibodies, HCV.SDS-PAGE denaturating : multiple protein bands 150 – 210 kDa
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: 0.2% Sodium Azide
    Constituents: 2% Methyl-mannoside, 0.1M PBS, pH 7.4

General Info

  • Alternative names
    • Carcinoembryonic antigen
    • Carcinoembryonic antigen-related cell adhesion molecule 5
    • CD66e
    • CEA
    • Ceacam5
    • CEAM5_HUMAN
    • DKFZp781M2392
    • Meconium antigen 100
    • OTTHUMP00000199032
    • OTTHUMP00000199033
    • OTTHUMP00000199034
    see all
  • Function
    Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
  • Tissue specificity
    Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 7 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
  • Cellular localization
    Cell membrane.
  • Information by UniProt

References

This product has been referenced in:
  • Li Y  et al. Carcinoembryonic antigen interacts with TGF-{beta} receptor and inhibits TGF-{beta} signaling in colorectal cancers. Cancer Res 70:8159-68 (2010). Read more (PubMed: 20889724) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us and your interest in our products.

The Carcino Embryonic Antigen CEA protein (ab742) is isolated from colon carcinoma liver metastatic tissue. The protein is therefore in its native form and should maintain the post...

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Thank you for your enquiry.

I can confirm that ab4451 and ab15987 have been tested and guarantee for use in sELISA together according to the datasheets. Theab742 Carcino Embryonic Antigen CEA protein is full length native protein so should b...

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Thank you for your enquiry. Unfortunately, we have not determined the isoelectric point or the sequence for this product. The product is purified from colon carcinoma liver metastatic tissue and the purity is 95% by SDS-PAGE. I hope this informa...

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Thank you for your enquiry. Products ab10036 and ab10039 work as a pair in sandwich immunoassay, but we have not tested them with ab742 antigen, so I am not able to comment about that. If you decide to go ahead and purchase the products, we enc...

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Thank you for your enquiry and patience. We unfortunately do not have any additional information with regards to the epitope specificity(which was given per ISOBM classification). NCA2 is nonspecific cross-reacting antigen 2. Ab742 is suitable as im...

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Thank you for your enquiry. For the following antibodies, epitope specificity is given per ISOBM classification and we have only following information: Ab10038 - Epitope specificity group IV, cross-reactive with NCA2. Ab10039 - Epitope specificity ...

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The CEA antigen is purified from a single patient source, colon carcinoma liver metastatic tissue, by immunoaffinity purification. Unfortunately we have not checked the presence of membrane anchor in the preparation.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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