Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Chicken
    • Molecular weight
      68 kDa
    • Additional sequence information
      Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containing 4 subunits each with a binding site for biotin.

Specifications

Our Abpromise guarantee covers the use of ab73656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Activity: Greater than 11 U/mg, 1 unit binds 1µg biotin.
  • Applications

    Western blot

    ELISA

  • Form
    Lyophilised
  • Additional notes
    For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    Preservative: None

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute in sterile 18MOhm/cm H2O at not less than 100µg/ml, or more than 10mg/ml solution.

General Info

  • Alternative names
    • AVD
    • AVID_CHICK
    • Avidin
    • LOC396260
  • Function
    The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
  • Tissue specificity
    Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
  • Sequence similarities
    Belongs to the avidin/streptavidin family.
    Contains 1 avidin-like domain.
  • Post-translational
    modifications
    N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • Anti-Avidin antibody (ab6675) at 1/1000 dilution + Natural chicken Avidin protein (ab73656) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References

ab73656 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab73656.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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