Natural Chicken Erythrocyte Histone tetramer (H3-H4)2 protein (ab82263)

Overview

  • Product name
    Natural Chicken Erythrocyte Histone tetramer (H3-H4)2 protein
  • Protein length
    Full length protein

Description

  • Nature
    Native
  • Amino Acid Sequence
    • Species
      Chicken

Specifications

Our Abpromise guarantee covers the use of ab82263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.
    The tetramers have been prepared from pure histone octamers that have been crystallized and the structure published to 1.90 Amstrongs resolution. The requirement for high yields of ultra pure proteins for X-ray crystallography studies and mass-spectrometry studies has stimulated a development programme to purify proteins from the nuclei of chicken erythrocytes under very gentle conditions. Thus native Histone tetramers (H3-H4)2 can be produced without denaturation, with all the naturally occurring post-translational modifications.
  • Form
    Liquid
  • Additional notes
    The Histone tetramers (H3-H4)2 should be thawed very slowly in a cold room to avoid precipitation of KCl / phosphate. For stability protein should be kept below 0° for storage and at 4° for usage.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 50mM Monobasic dihydrogen potassium phosphate, 50mM Dibasic monohydrogen potassium phosphate, 2.5mM Benzamidine Hydrochloride, 100mM Potassium chloride

Images

  • SDS page analysis of ab82263

References

This product has been referenced in:
  • Pupaibool J  et al. Characterization of the Pneumocystis carinii histone acetyltransferase chaperone proteins PcAsf1 and PcVps75. Infect Immun 81:2268-75 (2013). Read more (PubMed: 23569117) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab82263.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up