The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab63835 was highly purified from the supernatant of the culture of Clostridium botulinum type C by cation exchange, hydroxyapatite and gel filtration columns.
No toxic effect was observed in two mice injected with 5 ug of C3 enzyme, showing little contamination of lethal toxins in this preparation.
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
ab63835 can be used for studies on the Rho-dependent signaling pathways in animal cells in vivo and in vitro and for the study of ADP-ribosylation of small GTP proteins, such as Rho and Rac proteins.
Its activity tested as tested and stimulation of human keratinocytes in culture was observed at 50 ng/ml.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
RelevanceMono-ADP-ribosyltransferase C3 from Clostridium botulinum D phage (Clostridium botulinum D bacteriophage). ADP-ribosylates eukaryotic Rho and Rac proteins on an asparagine residue. It has a Mass of 27.8kDa.
Natural Clostridium botulinum Exoenzyme C3 protein images
SDS-PAGE - Clostridium botulinum Exoenzyme C3 full length protein (ab63835)
ab63835 on SDS-PAGE (CBB staining). Molecular Weight: ~27.8 kDa.
References for Natural Clostridium botulinum Exoenzyme C3 protein (ab63835)
has not yet been referenced specifically in any publications.
Publishing research using ab63835? Please let us know so that we can cite the reference in this datasheet.
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