Natural Cow 160 kD Neurofilament Medium protein (ab73759)

Overview

  • Product name
    Natural Cow 160 kD Neurofilament Medium protein
  • Protein length
    Full length protein

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Cow
    • Molecular weight
      160 kDa
    • Additional sequence information
      Source = bovine spinal cord

Specifications

Our Abpromise guarantee covers the use of ab73759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 6M Urea, 2mM DTT, 10mM Sodium phosphate, 1mM EDTA, pH 7.5

  • Reconstitution
    Reconstitute in sterile 18MO-cm H2O. Upon reconstitution NRFab73759 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

General Info

  • Alternative names
    • 150kDa medium
    • NEF3
    • NEFM
    • Neurofilament 3
    • NF160
    • NFM
    see all
  • Relevance
    Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • Cellular localization
    Cytoplasm

References

ab73759 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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