Natural Cow 160 kD Neurofilament Medium protein (ab73759)

Overview

  • Product nameNatural Cow 160 kD Neurofilament Medium protein
  • Protein lengthFull length protein

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesCow
    • Molecular weight160 kDa
    • Additional sequence informationSource = bovine spinal cord

Specifications

Our Abpromise guarantee covers the use of ab73759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Western blot

  • Purity> 95 % SDS-PAGE.
    Purity is greater than 98.0% as determined by RP-HPLC and SDS-PAGE.
  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 6M Urea, 2mM DTT, 10mM Sodium phosphate, 1mM EDTA, pH 7.5

  • ReconstitutionReconstitute in sterile 18MO-cm H2O. Upon reconstitution NRFab73759 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

General Info

  • Alternative names
    • 150kDa medium
    • NEF3
    • NEFM
    • Neurofilament 3
    • NF160
    • NFM
    see all
  • RelevanceNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.
  • Cellular localizationCytoplasm

Natural Cow 160 kD Neurofilament Medium protein images

  • All lanes : Anti-160 kD Neurofilament Medium antibody - Neuronal Marker (ab9034) at 1/5000 dilution

    Lane 1 : Natural Cow 160 kD Neurofilament Medium protein (ab73759) at 0.1 µg
    Lane 2 : Natural Cow 160 kD Neurofilament Medium protein (ab73759) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Natural Cow 160 kD Neurofilament Medium protein (ab73759)

ab73759 has not yet been referenced specifically in any publications.

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