Natural Cow Collagen I protein (ab7526)

Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Cow

Specifications

Our Abpromise guarantee covers the use of ab7526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Immunoprecipitation

    Western blot

  • Form
    Liquid
  • Additional notes

    This product has been prepared from Bovine Placenta and is chromatographically and immunologically pure. It reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

    This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: 0.01% Sodium Azide
    Constituents: 0.5M Acetic acid, pH 4.5

General Info

  • Alternative names
    • Alpha 1 type I collagen
    • Alpha 2 type I collagen
    • alpha 2 type I procollagen
    • alpha 2(I) procollagen
    • alpha 2(I)-collagen
    • Alpha-1 type I collagen
    • alpha1(I) procollagen
    • CO1A1_HUMAN
    • COL1A1
    • COL1A2
    • collagen alpha 1 chain type I
    • Collagen alpha-1(I) chain
    • collagen alpha-1(I) chain preproprotein
    • Collagen I alpha 1 polypeptide
    • Collagen I alpha 2 polypeptide
    • collagen of skin, tendon and bone, alpha-1 chain
    • collagen of skin, tendon and bone, alpha-2 chain
    • Collagen type I alpha 1
    • Collagen type I alpha 2
    • EDSC
    • OI1
    • OI2
    • OI3
    • OI4
    • pro-alpha-1 collagen type 1
    • type I proalpha 1
    • Type I procollagen
    • type I procollagen alpha 1 chain
    see all
  • Function
    Type I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificity
    Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in disease
    Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
    Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
    Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
    Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
    Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
    Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • Post-translational
    modifications
    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Images

  • ab7526 used to generate a standard curve for ELISA, diluted to a concentration of 4, 2, 1 and 0.5 ug.
    Samples were from bovine LD muscle.

    ab34710 was used at a 1/1000 dilution as the primary antibody.

    See Abreview

References

ab7526 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

The collagen protein ab7526 is purified from the full length native bovine protein by a process involving limited pepsin digestion. The exact purity for this product is not determined.

The bovine type I collagenab7526 is prepared by a proprietary method of limited pepsin digestion and selective salt precipitation and is supplied in a sodium acetate buffer containing sodium azide. However it is not tested to be free of telepeptides.

Application
ELISA

Review text: I used this collagen protein to generate my standard curve for ELISA, diluted to a concentration of 4, 2, 1, 0.5 ug
Sample: Cow Tissue sections (Beef LD muscle)
Specification: Beef LD muscle
Blocking step: BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Type: Sandwich (Capture)

Other product details
Incubation time: 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: carbonated coating buffer

Primary antibody (in addition to 'Collagen I protein')
Primary antibody: Abcam primary antibody: Anti-Collagen I antibody (ab34710)
Dilution: 1/1000

Secondary antibody
Name: Abcam antibody:(not specified yet)
Dilution: 1/1000

Additional data
Additional Notes: Primary antibody: Ab34710 rabbit polyclonal to collagen I (1:1000 in carbobate coating buffer) add 100 ul to wells and incubate at 37C for 2 h
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Abcam user community

Verified customer

Submitted Aug 12 2011

Thank you for your enquiry. It is a native protein extracted by pepsin digestion. The basic structure should still remain.

Thank you for your enquiry and your interest in our product. We recommend non-denaturing and non-dissociating conditions. Other conditions are not supported.

Thank you for your phone call. Ab7526 was tested in a sandwich ELISA. General Instructions for sandwich ELISA to quantify collagen in solution: 1. Coat the plate with unlabeled anti-collagen (type specific) at 10 mg/ml. 2. Use biotinylated an...

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