Natural Cow Collagen IV protein (ab7529)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesCow
    • Molecular weight161 kDa

Specifications

Our Abpromise guarantee covers the use of ab7529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Immunoprecipitation

    ELISA

  • FormLiquid
  • Additional notes

    This protein is isolated from bovine placenta. 

    This product reacts with anti-Collagen Type IV. Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 0.1M Sodium Acetate. pH 4.5

General Info

  • Alternative names
    • Arresten
    • CO4A1_HUMAN
    • COL4A1
    • COL4A1 NC1 domain
    • COL4A2
    • COL4A3
    • COL4A4
    • COL4A5
    • collagen alpha-1(IV) chain
    • Collagen IV Alpha 1 Polypeptide
    • Collagen IV Alpha 2 Polypeptide
    • Collagen Of Basement Membrane Alpha 1 Chain
    • Collagen Of Basement Membrane Alpha 2 Chain
    • Collagen Type IV
    • Collagen Type IV Alpha 1
    • Collagen Type IV Alpha 2
    • Collagen Type IV Alpha 3
    • Collagen Type IV Alpha 4
    • Collagen Type IV Alpha 5
    see all
  • FunctionType IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • Tissue specificityHighly expressed in placenta.
  • Involvement in diseaseDefects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
  • Sequence similaritiesBelongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • DomainAlpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • Post-translational
    modifications
    Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt

References for Natural Cow Collagen IV protein (ab7529)

ab7529 has not yet been referenced specifically in any publications.

Product Wall

The Collagen IV protein (ab7529) has been isolated from bovine placenta. This contains both alpha chains of a1(IV) and a2(IV).

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This protein (ab7529: Collagen IV protein) has been tested and characterized for WB, IP and ELISA. Although, the protein is in its native form (dissolved in 0.1M Sodium Acetate. pH...

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