Natural Cow Plasmin protein (ab93009)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesCow
    • Additional sequence informationSource = prepared from plasminogen

Specifications

Our Abpromise guarantee covers the use of ab93009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.
    Prepared from plasminogen by activation with immobilized Human uPA.100% functionally active plasmin is purified from the activation reaction by immobilized SBTI.
  • FormLiquid
  • Additional notesSolubility: > 2 mg/mL and < 5 mg/mL Spectrophotometric Data: Epsilon0.1% = 1.69
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 0.1M HEPES, pH 7.4

General Info

  • Alternative names
    • Plasminogen
    • PLG
  • RelevancePlasmin is an enzyme formed in the circulating blood, and many other extracellular fluids, from the zymogen plasminogen. Plasminogen is a single-chain glycoprotein with 790 amino acid residues. Activation to the active form, plasmin, by urokinase involves cleavage at the arg-val bond between residues 560 and 561, resulting in the formation of the 2-chain plasmin molecule held together by 2 disulfide linkages. The heavier chain contains about 411 residues and the lighter chain about 233. The main function of plasmin is the digestion of fibrin in blood clots. Plasmin is a proteolytic enzyme with a specificity similar to that of trypsin. Like trypsin, plasmin belongs to the family of serine proteinases, in which the active site catalytic triad, his-57, asp-102, and ser-195 (chymotrypsin numbering), is situated in the light chain. Plasmin acts as a proteolytic factor in a variety of processes other than fibrinolysis; including embryonic development, tissue remodelling, tumour invasion and inflammation; in ovulation it weakens the walls of the Graafian follicle. A Deficiency of plasmin may lead to thrombosis, as clots are not degraded adequately.
  • Cellular localizationSecreted

Natural Cow Plasmin protein images

  • 10% SDS-PAGE analysis of ab93009
    Lane 1: ab93009 (3 ug) Reduced
    Lane 2: ab93009 (3 ug) Non-reduced
    Lane 3: Molecular weight markers

References for Natural Cow Plasmin protein (ab93009)

ab93009 has not yet been referenced specifically in any publications.

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My contact at the lab sent the following information:

"The lower band is bovine midiplasmin. Partial cleavage of bovine p...

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