Natural Cow Thrombin protein (ab62389)

Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Cow
    • Additional sequence information
      Prepared from purified prothrombin using a modification of the Lundblad procedure as described by Nesheim et al. 1983.

Specifications

Our Abpromise guarantee covers the use of ab62389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form
    Liquid
  • Additional notes
    Concentration varies from lot to lot but is between 5-10mg/ml
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol

General Info

  • Alternative names
    • Coagulation factor II
    • Coagulation factor II thrombin
    • EC 3.4.21.5
    • F2
    • Factor II
    • Prepro coagulation factor II
    • Prothrombin
    • Prothrombin B chain
    • PT
    • RPRGL2
    • Serine protease
    • THPH1
    • THRB_HUMAN
    • Thrombin heavy chain
    see all
  • Function
    Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Factor II deficiency
    Ischemic stroke
    Thrombophilia due to thrombin defect
    Pregnancy loss, recurrent, 2
  • Sequence similarities
    Belongs to the peptidase S1 family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
  • Cellular localization
    Secreted, extracellular space.
  • Information by UniProt

References for Natural Cow Thrombin protein (ab62389)

ab62389 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Anfrage. Leider sind beide unserer Thrombin Proteine, purifizierte Proteine ohne Tag.  Wir haben daher nicht ein His-Tag-Thrombin aktuell in unserem Katalog. Ich werde jedoch mit meinen Kollegen schauen, ob wir in Planung haben ein...

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