Natural Human alpha 1 Antichymotrypsin protein (ab80517)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab80517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20mM Tris, 150mM Sodium chloride, pH 7.4

  • ReconstitutionReconstitute with deionized water.

General Info

  • Alternative names
    • SERPINA3
    • AACT
    • AACT_HUMAN
    • ACT
    • Alpha-1-antichymotrypsin His-Pro-less
    • Antichymotrypsin
    • Cell growth inhibiting gene 24/25 protein
    • Cell growth-inhibiting gene 24/25 protein
    • GIG24
    • GIG25
    • Growth inhibiting protein 24
    • Growth inhibiting protein 25
    • MGC88254
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3
    • Serine (or cysteine) proteinase inhibitor clade A member 3
    • Serine proteinase inhibitor clade A member 3
    • Serpin A3
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3
    • SERPINA3
    see all
  • FunctionAlthough its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • Tissue specificityPlasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • Involvement in diseaseDefects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Cellular localizationSecreted.
  • Information by UniProt

Natural Human alpha 1 Antichymotrypsin protein images

References for Natural Human alpha 1 Antichymotrypsin protein (ab80517)

ab80517 has not yet been referenced specifically in any publications.

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Abreviews
Application Functional Studies
Cathepsin G activity was measured spectrofluorometrically (Spectra Max Gemini EM) at 320 nm for excitation and 420 nm for emission using Abz-TPFSGQ-EDDnp at 15 µM final (Genecust) as the substrate in PBS at 37 °C. Cathepsin G at 1 µM was previously incubated for 10 min at 37°C with or without Human alpha 1 Antichymotrypsin protein (ab80517) at 1 µM or 0.5 µM in PBS.

"Valayer Alexandre
Centre d'Etude des Pathologies Respiratoires (CEPR)
INSERM U1100
Faculté de Médecine, Université F. Rabelais
10, boulevard Tonnellé
37032 Tours cedex "
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Ms. SECRETARIAT CEPR U1100

Verified customer

Submitted Jun 22 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"