Natural human alpha 1 Antitrypsin protein (ab91136)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab91136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityActivity: When tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inihibitory.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesProtein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.433

    Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Preservative: None
    Constituents: 300mM Sodium chloride, 30mM Sodium phosphate, pH 6.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • ReconstitutionOnce reconstituted, ab91136 is stable for one week at 4°C.

General Info

  • Alternative names
    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all
  • FunctionInhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificityUbiquitous. Expressed in leukocytes and plasma.
  • Involvement in diseaseAlpha-1-antitrypsin deficiency
  • Sequence similaritiesBelongs to the serpin family.
  • DomainThe reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    modifications
    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localizationSecreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

Natural human alpha 1 Antitrypsin protein images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1. 5 µg alpha 1 Antitrypsin (reduced/heated)
    Lane 2. 10 µg alpha 1 Antitrypsin (reduced/heated)
    Lane 3. 20 µg alpha 1 Antitrypsin (reduced/heated)
    Lane 4. Molecular weight markers
    Lane 5. 5 µg alpha 1 Antitrypsin (non-reduced/no heat)
    Lane 6. 10 µg alpha 1 Antitrypsin (non-reduced/no heat)
    Lane 7. 20 µg alpha 1 Antitrypsin (non-reduced/no heat)
  • Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) at 1 µg/ml + Natural human alpha 1 Antitrypsin protein (ab91136) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

References for Natural human alpha 1 Antitrypsin protein (ab91136)

ab91136 has not yet been referenced specifically in any publications.

Product Wall

The protein concentration of lot GR 108058 was 3.79 mg/ml. Each mg was lyophilized from 264 ul of buffer and you should reconstitute with 264 ul of deionized water in order to maintain a buffer of 30 mM Na Phosphate and 300 mM NaCl.

Thank you for your enquiry.

We do not have set expiration dates for our products. Most proteins are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage in...

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Thank you for your reply.

Unfortunately we only have the testing information for the porcine pancreatic trypsin. It may work with human or bovine, but we just don't know for sure since this hasn't been tested.

I apologize that thi...

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Thank you for contacting us.

When ab91136 was tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inihibitory.

I hope this i...

Read More

Vielen Dank für Ihren Anruf.

Das lyophilisierte Protein sollte bei -20C gelagert werden. Wenn das Protein mit deionisierten Wasser resuspendiert wurde, lagern Sie es bitte in Aliquotesbei -80C.

Der EC ist übrigensfür...

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Thank you for contacting us.

The original concentration of the lot you received was 2.3 mg/ml, or 1 mg in 434.8 ul.

So, adding 434.8 ul of de-ionized water will reconstitute the constituents to their original concentrations and pH...

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I can confirm that the ab77937 is also glycosylated. I hope tthis information will be helpful.

Thank you for your email. ab91136 is indeed glycosylated. I have asked our lab for confirmaiton about ab77937 also; I will forward you the information soon. I hope this will help.

Thank you for contacting us. The only difference in ab91136 and ab77937 is that ab91136 is active form of protein; which means this protein is fully guaranteed to be used in functional assays. We unfortunately do not have products related to U3...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"