Natural Human alpha 2 Macroglobulin protein (ab77935)



  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman


Our Abpromise guarantee covers the use of ab77935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot


  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesAll human source materials have tested negative for HIV 1, HIV 2 and tested non-reactive for anti-HCV, anti-HBc antibodies and HBsAg by FDA required tests. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 1mg Glycine, 30mM Sodium phosphate, pH 7.0

  • ReconstitutionReconstitute with 483 µl distilled water. Concentration 2.07 mg/ml (prior to lyophilization).

General Info

  • Alternative names
    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • Tissue specificitySecreted in plasma.
  • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • Cellular localizationSecreted.
  • Information by UniProt

Natural Human alpha 2 Macroglobulin protein images

  • Anti-alpha 2 Macroglobulin antibody (ab58703) at 1 µg/ml + Natural Human alpha 2 Macroglobulin protein (ab77935) at 0.1 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 3 minutes

References for Natural Human alpha 2 Macroglobulin protein (ab77935)

This product has been referenced in:
  • Tasnim F & Zink D Cross talk between primary human renal tubular cells and endothelial cells in cocultures. Am J Physiol Renal Physiol 302:F1055-62 (2012). Read more (PubMed: 22319059) »

See 1 Publication for this product

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