Natural Human alpha 2 Macroglobulin protein (ab91104)

Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman

Specifications

Our Abpromise guarantee covers the use of ab91104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity> 95 % SDS-PAGE.

  • FormLyophilised
  • Additional notesProtein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.81. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    Preservative: None
    Constituents: Glycine, 30mM Sodium phosphate, pH 7.0

General Info

  • Alternative names
    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • Tissue specificitySecreted in plasma.
  • Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • Cellular localizationSecreted.
  • Information by UniProt

Natural Human alpha 2 Macroglobulin protein images

  • SDS-PAGE: 4-12% Bis-Tris-HCl NuPAGE gel
    Lane 1. 5 µg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 2. 10 µg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 3. 20 µg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 4. 5 µg alpha 2 Macroglobulin (reduced/ heated)
    Lane 5. 10 µg alpha 2 Macroglobulin (reduced/ heated)
    Lane 6. 20 µg alpha 2 Macroglobulin (reduced/ heated)
    Lane 7. Molecular weight markers

References for Natural Human alpha 2 Macroglobulin protein (ab91104)

ab91104 has not yet been referenced specifically in any publications.

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