Natural Human Apolipoprotein B (ab77859)



  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman


Our Abpromise guarantee covers the use of ab77859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activityCross-reacts with anti-LDL.
  • Applications

    Western blot

  • FormLiquid
  • Additional notesCross-reacts with anti-LDL.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 200mM Tris, 10mM Sodium decyl sulfate, pH 8.25

General Info

  • Alternative names
    • Apo B-100
    • Apo B-48
    • APOB
    • ApoB 100
    • ApoB 48
    • Apolipoprotein B
    • Apolipoprotein B 100
    • Apolipoprotein B-48
    • Apolipoprotein B48
    • FLDB
    • LDLCQ4
    see all
  • FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in diseaseHypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similaritiesContains 1 vitellogenin domain.
  • Post-translational
    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localizationCytoplasm. Secreted.
  • Information by UniProt

References for Natural Human Apolipoprotein B (ab77859)

ab77859 has not yet been referenced specifically in any publications.

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The gel that was used was Tris-Acetate. We use Invitrogen's NuPAGE 3-8% TA gels:
I hope this information is helpful to you. Please do not hesitate to co...

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I have contacted the lab asking for the protocol used to produce the WB shown on the datasheet. Here is the answer I got:
Ab77859 was used at 0.01 ug as shown in the WB. When we used the protein at higher concentr...

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For ab77859: Apo B-100 is isolated from other smaller apoproteins by gel filtration chromatography. The presence or absence of these smaller apoproteins is tested by Ouchterlony immunodiffusion using the correspon...

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I am a little concerned about the formulation of ab77859. It is in 200mM Tris, 10mM Sodium decyl sulfate, pH 8.25, and I am wondering if the sodium decyl sulfate concentration is sufficient to denature the protein, possibly making it incompatible with ...

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Thank you for the clarification. I had a feeling it would not be that simple! I expect that the buffer supplied in the kit for diluting experimental samples is suitable for standards too. You might ask the kit supplier if their standard is equivalent t...

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Thank you for bringing this to our attention. The product, apolipoprotein B protein, has not, to our knowledge, been tested in ELISA. The information we have from the laboratory that provides the protein to us is as follows: "ApoB-100 (MW 413kDa) is is...

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Thank you for your enquiry and your interest in our products. I can confirm that all these 3 products (ab77859, ab77903, ab82277) do not have Apo B-48. I hope this information will be useful for you.

Thank you for your enquiry. The actual purity has not been determined for ab77859, Apolipoprotein B. However the following information may be helpful. ab77859 is manufactured to eliminate all other apoproteins including B48. To manufacture this...

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